Over-expression of a LEA gene in rice improves drought resistance under the field conditions | Semantic Scholar (2024)

The expression patterns of LEA genes were investigated in two advanced mutant rice genotypes subjected to the drought stress condition and different physiological traits including photosynthetic rate, leaf chlorophyll content, and photosystem II photochemical efficiency were analyzed to confirm their drought tolerance.

It is demonstrated that root-specific overexpression of OsNAC10 enlarges roots, enhancing drought tolerance of transgenic plants, which increases grain yield significantly under field drought conditions.

It is demonstrated that OsGF14b is involved in the drought resistance of rice plants, partially in an ABA-dependent manner.

Overexpression of OsDIL significantly enhanced drought resistance in transgenic rice during reproductive development, while showing no phenotypic changes or yield penalty under normal conditions, making it an excellent candidate gene for genetic improvement of crop yield in adaption to unfavorable environments.

The co-overexpression of two genes, encoding a constitutively active form of a bZIP transcription factor and a protein kinase involved in the abscisic acid signaling pathway, showed significantly enhanced drought resistance compared with the single-gene transgenic lines and the negative transgenic plants.

It is reported that rice OsOFP6 regulates growth and development, and alters responses to drought and cold stresses, and new insights are provided into the multiple functions of OVATE family proteins.

Findings demonstrated that OsNAC6 might be a candidate of stress-responsive NAC regulatory gene that can be used to develop either drought or salt tolerant tolerant transgenic plants.

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It is shown that overexpression of stress responsive gene SNAC1 (STRESS-RESPONSIVE NAC 1) significantly enhances drought resistance in transgenic rice in the field under severe drought stress conditions at the reproductive stage while showing no phenotypic changes or yield penalty.

This study provides direct evidence supporting the hypothesis that LEA proteins play an important role in the protection of plants under water-or salt-stress conditions and finds that the extent of increased stress tolerance correlated with the level of the HVA1 protein accumulated in the transgenic rice plants.

Transient assay of beta-glucuronidase (GUS) activity in barley aleurone cells shows that, coupled with ABRC1, the shortest minimal promoter (Act1-100P) gives both the greatest induction and the highest level of absolute activity following ABA treatment.

It is shown here that overexpression of the cDNA encoding DREB1A in transgenic plants activated the expression of many of these stress tolerance genes under normal growing conditions and resulted in improved tolerance to drought, salt loading, and freezing.

OsDREB1A is potentially useful for producing transgenic monocots that are tolerant to drought, high-salt, and/or cold stresses and has functional similarity to DREB 1A, however, in microarray and RNA blot analyses, some stress-inducible target genes of the DREb1A proteins that have only ACCGAC as DRE were not over-expressed in the OsDRE B1A transgenic Arabidopsis.

Both the salt-induced endogenous ABA levels and a greater molecular response of root tissue to ABA were associated with the varietal differences in tolerance.

It is suggested that osmotic potential might be a major factor in regulating the expression of kin1 and cor6.6 during several developmental processes.

The transgenic tobacco with Prd29A/TSP demonstrated TPS expression with increased drought tolerance under drought stress and some obvious morphological changes including dwarf and fine shoot, lancet_shaped leaves and vigorous auxiliary buds have been observed in a few transformed plants.

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